Application specific products
Products for Bioanalytical Applications
> Isolute PPT+ Protein Precipitation Plates
> Isolute SLE+ Supported Liquid Extraction Plates
> Evolute ABN
Products for Forensic Applications
> Isolute Confirm HCX
> Isolute 101
> Isolute THC
> Isolute Confirm HAX
> Isolute HM-N
> Products for Doping Control
Products for Environmental Applications
> Isolute ENV+
> Isolute Layered SPE Columns
> Isolute EPH
> Isolute PAH
> Isolute PAH HC
> Isolute Triazine
> Isolute TPH
> Isolute O&G
Products for Agrochemical & Food Applications
> Isolute HM-N
> Isolute SAX/PSA
> Isolute Multimode SPE Columns
> Isolute ENV+
Products for Bioanalytical Applications
In today’s bioanalytical laboratory, reliable, high throughput sample preparation techniques are essential. IST Sample Preparation Products from Biotage provide a range of solutions matching the requirements for throughput and sample clean-up throughout the drug development process. Biotage is dedicated to developing sample preparation products that solve the daily sample preparation challenges faced by bioanalytical scientists.
ISOLUTE PPT+ Protein Precipitation Plates
ISOLUTE PPT+ Protein Precipitation Plates provide effective, high throughput, protein removal from plasma samples. The simple filtration based procedure is easily automated, and is applicable to a wide range of drugs and metabolites.
ISOLUTE SLE+ Supported Liquid Extraction Plates
ISOLUTE SLE+ Supported Liquid Extraction Plates are optimized for reliable high throughput extraction of 96-samples simultaneously using liquid handling systems. Up to 400µL of aqueous sample can be extracted per extraction well. For larger sample volumes, ISOLUTE HM-N columns for supported liquid extraction are available.
EVOLUTE ABN
EVOLUTE ABN SPE columns and plates are ideal for extraction of a broad range of compounds from biological fluid samples. The sorbent has been specifically designed for LC-MS assays, and has an optimized pore size distribution ensuring reduced ion suppression (improved signal to noise) for biological fluid extracts, compared to other resin based sample preparation products.
Products for Forensic Applications
Biotage produces a range of products for use in the area of forensic toxicology and doping control in animal and human sports.
ISOLUTE Confirm HCX Columns for High Extract Purity
Extract basic drugs from biological fluids with ISOLUTE Confirm HCX mixed mode SPE columns that provide two primary retention mechanisms, non-polar (C8) and strong cation exchange. This dual retention mechanism allows a rigorous interference elution regime to be used without reducing recovery of the analytes of interest when compared to non-selective LLE, resin or C18 based methodologies.
Application Notes for Isolute HCX columns (downloadable after registration) :
- Amphetamine and methamphetamine (IST1034)
- Barbiturates (IST1032)
- Cocaine and benzoylecgonine (IST1002)
- Opiates (codeine and morphine) (IST1050)
- LSD (IST1041)
- Phencyclidine (IST1039)
- Fractionation of basic, neutral and acidic drugs of abuse (IST1044)
Toxicological Screening Using ISOLUTE 101 SPE Columns
ISOLUTE 101 is a non-selective polymeric sorbent used in toxicological screening for a wide range of drug classes from biological fluids. The 500 mg/6 mL column configuration (part number 101-0050-C) has been successfully applied to screening more complex samples such as post-mortem blood and tissue. (ref. Stimpfl et al, J. Anal. Toxicol., 2001, 25 (2): 125-129).
ISOLUTE THC SPE Columns
ISOLUTE THC SPE Columns for the extraction of 11 Nor-∆9-THC-9-carboxylic acid from urine. ISOLUTE THC columns are designed for the rapid, cost effective extraction of the THC carboxylic acid metabolite from urine prior to confirmation analysis by GC-MS. The robust procedure, based on a non-polar retention mechanism, is our method of choice for THC metabolite confirmation testing.
ISOLUTE Confirm HAX SPE columns
Ideal for high purity extraction of acidic drugs from biological fluids, ISOLUTE Confirm HAX SPE columns contain both non-polar (C8) and strong anion exchange (–NR3+) functional groups.
ISOLUTE HM-N Supported Liquid Extraction (SLE) Columns
Supported liquid extraction is a popular approach for drugs of abuse extraction in forensic analysis. Optimized to provide high recoveries from biological fluids, ISOLUTE HM-N columns are packed with a modified form of diatomaceous earth. Designed for processing under gravity, these simple to use columns are a high productivity alternative to liquid-liquid extraction.
Products for Doping Control Applications
ISOLUTE C8, C18 and ENV+ columns are ideal for applications in doping control in sport such as the extraction of steroids and their glucuronide and sulphate metabolites from human urine. For example, ISOLUTE C8 is used to extract 5 α−dihydrotestosterone from athlete’s urine (ref. Kicman et al, Clinical Chemistry, 1995, 41/11, 1617-1627).
Products for Environmental Applications
Biotage produces a range of products for use in the area of environmental research.
ISOLUTE ENV+ SPE columns
Efficiently extract polar analytes from water samples using ISOLUTE ENV+ SPE columns. This hyper-crosslinked polystyrene-divinylbenzene polymer based sorbent has a wettable, hydroxyl modified surface with an extremely high area (>1000 m2/g). The sorbent is optimized for loading large volume samples, and high analyte recoveries are achieved when loading at fast flow rates of 60 mL/min.
Typical recoveries (% absolute) of polar herbicides using ISOLUTE ENV+
Dicamba : 98%
Mecoprop :103%
MCPA : 93
2, 4-D : 91
Bromoxynil : 101
2, 4-DB : 104
Bentazone : 90
Ioxynil : 104
Benazolin : 95
> 1 L water sample spiked at 100 ppb for each analyte, extracted using ISOLUTE ENV+ 200mg/6 mL column
ISOLUTE Layered SPE Columns for Extracting Multiple Analyte Suites
Ideal for applications where analytes with a broad range of polarity are to be extracted from a single sample. ISOLUTE layered SPE columns extend the range of analytes extracted using a single column. Extract a range of endocrine disrupters or combined suite of organochlorine, triazine and organophosphorus pesticides simultaneously with the layered columns. Costs can be reduced and productivity improved as fewer samples need to be prepared for each assay.
Layered columns available :
- ISOLUTE C2/C18(EC) 500 mg/3 mL
- ISOLUTE C2/C18(EC) 1 g/6 mL
- ISOLUTE C8/ENV+ 400 mg/6 mL
- ISOLUTE C18/ENV+ 400 mg/6 mL
ISOLUTE EPH Columns
ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8 - C40 aliphatics, C10 – C22 aromatics). The columns consist of a proprietary blend of silica/alumina that has been optimised to achieve a clean fractionation of the aliphatic / aromatic EPH components. ISOLUTE EPH columns have been designed to eliminate the common problem of aromatic breakthrough into the aliphatic fraction. The approach is broadly based on procedures published by the Massachusetts Dept of Environmental Protection (MADEP) and the TPH Criteria Working Group (TPHCWG). The manual ISOLUTE EPH column uses a similar fractionation column sorbent mass to these published methods whereas the automated ISOLUTE EPH product and associated methodology provides reliable high throughput fractionation with substantial reductions in solvent consumption and costs, in some cases eliminating the need for evaporation of aliphatic and aromatic fractions prior to analysis.
ISOLUTE PAH SPE columns
ISOLUTE PAH SPE columns are designed to extract PAHs from water samples containing polar interferences such as humic acids. The layered column contains a NH2 layer above a C18 sorbent bed. Polar interferences are retained on the NH2 layer during extraction, but are not eluted with the PAHs, giving a pure extract for analysis.
ISOLUTE PAH HC SPE Columns
Rapidly clean up hexane extracts of PAHs from oil contaminated soils with ISOLUTE PAH HC SPE columns.
ISOLUTE Triazine SPE columns
ISOLUTE Triazine SPE columns are optimized for reliable, reproducible extraction of triazine pesticides and their polar metabolites from water samples. Each batch of ISOLUTE Triazine is QC checked for recoveries of the atrazine–desethyl and–desisopropyl metabolites.
ISOLUTE TPH SPE Columns
Extract total petroleum hydrocarbons from water samples with ISOLUTE TPH SPE columns. The simple method eliminates tedious sample shaking and emulsion formation problems associated with liquid-liquid extraction.
ISOLUTE O&G SPE Columns
ISOLUTE O&G columns are designed for extraction of total Oil & Grease from water samples, and allow fractionation of the sample into hexane extractable material (HEM) and silica gel treated hexane extracted material (SGT-HEM). This eliminates the need for separate liquid-liquid extraction and silica column fractionation steps.
Products for Agrochemical & Food Applications
Sample preparation in food and agrochemical applications is often complicated by the nature of the original sample matrix. In residue analysis, common sample types such as fruit, vegetables, soil or tissue are solid or semisolid, and need specific sample pre-treatment methods to allow extraction of analytes. Some liquid samples, such as milk, have high fat concentrations, making them difficult to handle using traditional methods. In addition, the complexity of these matrices requires additional clean-up to remove interferences prior to analysis, particularly for low level residue work. This section highlights a range of products and techniques for simplifying sample handling and clean-up of these difficult samples.
ISOLUTE HM-N Columns for Supported Liquid Extraction
ISOLUTE HM-N Supported Liquid Extraction columns provide a simple alternative to traditional liquid-liquid extraction for complex aqueous matrices such as milk. The columns contain a modified form of diatomaceous earth, which acts as an inert support for the extraction process. The aqueous sample is loaded under gravity, and is absorbed onto the support. When a suitable water immiscible solvent is passed through the column, the analytes are efficiently desorbed, and the extract is collected. As the aqueous and solvent phases are never in direct contact, emulsion formation, a particular problem for fat laden samples, is eliminated.
ISOLUTE SAX/PSA SPE Columns for Interference Removal
Remove polar interferences and pigments from plant extracts prior to pesicide residue analysis with ISOLUTE SAX/PSA columns.
ISOLUTE Multimode SPE Columns for Acrylamide Clean-up
Determination of acrylamide in cooked foodstuffs such as fried potato products can be problematic due to the highly polar, water soluble nature of the analyte, and the complexity of the sample matrix. ISOLUTE Multimode SPE columns provide an extremely effective clean-up of aqueous food extracts, allowing acrylamide determination by LC-MS.
ISOLUTE ENV+ SPE Columns for Extraction of Highly Polar Compounds
ISOLUTE ENV+ is an extremely retentive SPE sorbent used for extraction of highly polar pesticides and herbicides from aqueous samples. Unlike other polymeric SPE sorbents, it is able to retain highly water soluble compounds such as acrylamide from aqueous food extracts.