CHROMOSOME KIT and Medium P
The Chromosome Kit P was developed to optimize the preparation of cultures of peripheral blood lymphocytes for subsequent chromosome analysis.
Special attention has been paid to the sample / medium volume with respect to the size of the culture surface area which together with the particular composition of the culture medium leads to an optimal mitotic index.
The Chromosome Kit P is a sterile complete medium (there is no requirement to add other components) which is supplied ready to use in a TC test tube. Its special formulation also makes it suitable for cultures of foetal blood lymphocytes obtained via cordocentesis.
CHROMOSOME KIT and Medium M
The Chromosome Kit M was developed to optimize the preparation of bone marrow cell cultures for subsequent chromosome analysis.
The use of the Chromosome Kit M test tube allows a substantial reduction in processing and handling procedures, which directly benefits laboratory routines.
The use of Chromosome Kit M results in simplified working protocols, and guarantees that microscopic observations are performed on reproducible and high quality samples: the number of metaphases and chromosome quality are in fact significantly higher when compared to those obtained by the use of either conventional media or with other commercial kits.
Cell synchronization using SYNCHROSET
In order to obtain chromosome preparations with a high number of metaphases and outstanding quality, the use of Synchroset, in combination with either Chromosome Kit P or Chromosome Kit M is recommended.
Using the two solutions, "A" and "B", which are provided as part of the kit, and an extremely simple protocol, synchronization of the cell cycle in both lymphocytes and bone marrow cells becomes straightforward.
The Synchroset may also be used for the production of high numbers of prometaphases in which the chromosomes are suitable for high resolution banding techniques.
In these preparations the individual bands normally visualized with standard methods may be divided into sub-bands (up to 850-1.000 per haploid set).
This methodology is suitable for more precise identification of breakpoints and minor abnormalities such as micro deletions, which often cause severe plurimalformative conditions.
Amniomed PLUS medium for Foetal Cells
Amniomed Plus provides enhanced plating and efficiency in prenatal cell culture.
The new improved formulation optimizes the primary in vitro culture of foetal human cells from amniotic fluid and chorionic villi.
The Optichrome has been specifically designed to optimize the environmental conditions in which the final evaporation of the methyl acetate or ethyl acetate fixative takes place during the metaphase.
These environmental conditions have a huge effect on the quality of the chromosome analysis preparations.
The Optichrome will ensure excellent results, perfectly reproducible in any situation.