Targeted Sample Enrichment
Achieve efficient and consistent coverage of your region of interest with KAPA Long Range HotStart DNA Polymerase. High yield amplification from complex targets up to 20 kb with minimal optimization.
Pooled Amplicon Libraries for Resequencing
The ultra-high fidelity and high yields of KAPAHiFi HotStart DNA Polymerase is critical for minimizing PCR errors and increasing coverage when pooling amplicon libraries for resequencing projects using next-generation DNA sequencing.
Library Quantification
Eliminate the need for expensive and time consuming titration runs – use qPCR as a fast and reliable method for quantifying libraries prior to emPCR (Roche 454 FLX/Titanium and ABI SOLiD) or bridge PCR (Illumina Genome Analyzer). Accurate quantification of complex genomic libraries can be difficult due to inherent amplification biases of the DNA polymerase used in qPCR. The new KAPA NGS library quantification kits contain an engineered DNA polymerase that is capable of more balanced amplification across both high GC and AT-rich genomes leading to more reliable quantification.
Genome Closure and Finishing
The relatively short read lengths of NGS platforms make whole genome assembly more problematic and result in more sequence gaps than traditional Sanger sequencing methods. Without subclones, next-generation whole genome sequencing requires PCR for finishing and sequence gap closure. KAPAHiFi HotStart is recommended when finishing PCR products are sequenced using NGS. Ultra-high fidelity is important since all NGS templates are clonally amplified and are therefore more sensitive to errors introduced by PCR.
For sequence gaps >5 kb, KAPA HiFi HotStart and KAPA Long Range HotStart are recommended.
If the sequence context around the gaps is difficult to PCR due to high GC content or secondary structure, KAPA2G Robust HotStart is recommended. The higher processivity of the engineered KAPA2G Robust DNA Polymerase makes this the enzyme of choice for amplifying difficult templates.
