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Evalution multiplex analysis platform for biomarkers

Client: MyCartis

Evalution – the multiplexed analysis of biomarkers made fast and simple

EvalutionAccelerate your research, trust your results
Evalution is MyCartis’ multiplex analysis platform tailored to the development of clinical biomarkers. It has been designed to analyze a broad range of protein and nucleic-acid based biomarkers to delivers high quality data and rapid results from minimal handling steps with unprecedented ease. Evalution integrates into a single instrument all the functions of incubation, washing, and optical readout for seamless operation of sophisticated assay protocols. The combination of customizable encoded microparticles with microfluidic assay plates in Evalution enables the combined analysis of protein and nucleic-acid biomarkers simultaneously.

With Evalution :

  • Researchers and developers fasten the validation of biomarker panels and the translation into clinical use.
  • Operators improve efficiency in the routine execution of assays through simple and fast procedures

 

  • ↓ MyCartis Evalution Brochure

    ↓ Evalution Technical Note

    ↓ Multiplex immunoassay development Technical Notes

    ↓ Lightening the black box Technical Note

    ↓ Serology testing Technical Note

  • Available soon

  • Encoded microparticles

    Produced in MEMS fab from well-established silicon machining technology.Microparticles

    Thousands of digital codes available for high multiplexing capabilities.

    Compatible with wide range of surface chemistries for multifunctional usage.

    High reproducibility for high quality data.

     

    Microfluidic assay plate

    MicrofluidicIntegrated reaction and detection environments to ensure short time to results with minimal handling steps.

    Microfluidic environment for ultra-fast biological reactions.

    Ability to run from 1 to 16 samples at the same time.

    Small volumes to preserve precious samples and reagents.

     

    Instrument

    • Full temperature (25-95 °C) and pressure control for flexible and reliable assays.
    • Highly sensitive optics compatible with Phycoerythrin (PE) fluorophores for femtogram/ml level of detection.
    • Real-time visualization of reactions and full data traceability for easy and reliable assay development.
    • Separate preparation station for the loading of custom set of encoded microparticles into the assay plates.

     

    Software

    • User-friendly interface with drag and drop functionality for fast and easy editing of protocol.
    • On-the-fly adjustment of protocol for fast assay optimisation.
    • Real-time building of graphs for full insight into the reaction.
    • Comprehensive data analysis for biomarker quantification.

     

    Evalution application capabilities

    Evalution has the ability to perform both protein and nucleic acid assays in fast and simple formats. This versatility makes the platform usable in a wide range of application areas.

    Nucleic acid applications

    Qualitative applications

    • Species detection
    • Genotyping
    • SNP detection
    • Melt curve analysis

     

    Quantitative applications

    • mRNA profiling
    • miRNA profiling
    • Load detection/monitoring

     

    Protein applications

    • Sandwich immunoassay
    • Competitive immunoassay
    • Antibody capture
    • Antibody selection
    • Binding kinetics
    • Reverse protein assay

     

    Fast and simple assays

    Running a multiplexed assay on Evalution is fast and simple. The integration of incubation, washing, and optical readout into one platform results in sophisticated, lengthy protocols being reduced to contain only a few handling and pipetting steps. For instance the quantification of low-abundant protein targets is performed in less than two hours while the detection of point mutations in amplicons is performed within 15 minutes.
    Fast and simple assays provide developers with actionable information rapidly to speed up the development cycle and save labor time.

    Key benefits of Evalution

    • Powerful tool for assay development.
    • Broad range of applications.
    • Single platform for protein and molecular assays.
    • Simple workflow with minimal handling.
    • High sensitivity and broad dynamic range.
    • Competitive cost per data point.

     

    Watch Evalution workflow

    Click here to watch the video of a typical workflow to execute a multiplexed sandwich immunoassay from prepared set of encoded microparticles. The reporting molecules are flushed together with the sample for a single-injection, wash-free protocol with real-time visualization of binding reactions. More traditional protocol with sequential steps of incubation, wash, and end-point readout are also possible.

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Evalution Platform explained

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OUR MOTIVATION

Many established technology providers learned the hard way that having a worldwide direct sales force, is often not the best solution. Now that the focus might have shifted to other continents, the cost of local affiliates weigh heavily on their organizations. For new and upcoming players, the investments are too high and the local barriers too complex. For innovative suppliers in general, the typical resellers and large catalog companies will lack the scientific knowhow and focus in order to be successful. New innovative suppliers need to have a fast and efficient access to the world.

OUR VISION

A local partner willing to go the extra mile, can offer the best of both worlds. We envisioned that by delivering more than what is expected of a distributor, it is possible to become a true local sales and marketing partner for our suppliers. With sales and service engineers trained to the same level of a direct sales force, both the customers and suppliers win. For our instrumentation suppliers, a strong service team is an essential driver for success. For reagents and consumables, we can ensure next-day deliveries to our endusers thanks to our centralized operations with local stock.

OUR MISSION

Serving laboratories in the Benelux, Sopachem thrive to be such kind of distributor. To maintain our focus, we created several divisions, each serving their specific markets, each with team of high level product and application specialist behind them. For our suppliers, our mission is to be the best sales and marketing partner available in our region, bringing the word distributor to another level. For our customers, it is our mission to serve them with highest standards of product knowhow, understanding their needs and applications. Be it by offering the best service or by introducing innovative technologies that will in the end help them advance their scientific work.
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