Discover the SunScript Reverse Transcriptases from Sygnis
SunScript Reverse Transcriptase RNaseH+ is a reverse transcriptase that has been engineered for high thermostability based on the well characterized HIV-1 RT. The enzyme is active up to temperatures of 85°C. It has RNA and DNA-dependent polymerase activity as well as RNaseH activity.
SunScript Reverse Transcriptase RNaseH- is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. It has RNA and DNA-dependent polymerase activity. The enzyme is active up to temperatures of 85°C. The elimination of RNaseH activity provides this enzyme with the best performance to allow high yields of full lenght cDNA synthesis on long RNA molecules with complex secondary structures.