Chromatrap for chromatin immunoprecipitation

Chromatrap® kits use revolutionary spin columns or microplates which contain discs of an inert, porous polymer to which protein A or G has been covalently attached. This patented format is unique. During an assay, the chromatin/antibody complex is selectively retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory:

• ChIP in under 5 hours from chromatin preparation to qPCR
• Less manual handling, eliminating sample loss through multiple pipetting steps
• Single column and 96-well formats available
• Optimised for 1000ng sample sizes: better results from smallersample
• Great results for samples between 50ng and 3000ng

Chromatrap® outperforms other chromatin immunoprecipitation methods:

Robust signal to noise

• Typically 2-3 times better than standard methods
• Low non-specific binding of the inert Chromatrap® discs
• No pre-blocking step required
• No DNA clean-up required

High surface area and excellent molecular mixing, ideal for:

• Low abundant targets
• Challenging cell types, including primary cells

Excellent ‘flow through’ characteristics of the column

• Easy, fast washing and elution steps during the assay
• Ensures highest levels of chromatin capture and release

Wide dynamic range

• From 50ng to 7000ng

Smaller sample sizes

• More ChIP assays from a single sample