The GenapSys Sequencer is based on a novel Sequencing-by-Synthesis chemistry which employs electrical-based detection of single nucleotide incorporations. Sequencing is carried out on CMOS microfluidic chips that have a scalable number of detectors : 1 million, 16 million or 144 million sensors. The 3 chips enable a wide range of throughputs suitable for various applications, providing flexibility without changing the underlying instrument. Sequence anything from cancer panels to whole human genomes simply by loading the right chip.
For example, the 16M sensor chip is capable of generating 1.2-2.0 Gb of high quality sequence data with average read lengths of >150 bp.
GenapSys has developed a novel electrical-based platform capable of accurately detecting single base incorporations. On the surface of the CMOS sequencing chip, there are millions of sensors, each designed to capture one clonally amplified bead. Every sensor consists of electrodes in close proximity to each other, both of which contact the bead captured by that sensor.
Since nucleotide incorporation into a growing DNA strand increases the amount of charged molecules around the bead, it changes the impedance measured by the sensor. The magnitude of the impedance change is correlated to the total change in the charge of DNA molecules and their surrounding layers around the bead, which is correlated to the number of incorporated nucleotides.
The plot contains a representative example of acquired data showing the distribution of measured differential signal across multiple nucleotide flows for a single template sequence. Flows that are expected to have nucleotide incorporations are highlighted in red. Non-incorporating flows are shown in blue and do not deviate significantly from the baseline. The inferred sequence, including the number of nucleotides in each incorporation are shown below.
|1002470||GenapSys Sequencing Prep System||unit|
|1001977||G3 Sequencing Kit||1 run|
|1001996||G3 Amplification Kit||1 run|
Originally presented at ASHG 2019, this webinar shares the findings by Jackson Laboratory on their evaluation of GenapSys sequencing system on reproducibility, throughput, and accuracy.