KAPA PROBE FAST qPCR Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).
Optimized for versatility and speed – KAPA PROBE FAST qPCR Kits provide fast and reproducible results for genotyping, gene expression analysis, and multiplexing offering:
↓ KAPA PROBE FAST Universal TDS
KK4701 | KAPA PROBE FAST Universal 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). |
KK4702 | KAPA PROBE FAST Universal 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). |
KK4703 | KAPA PROBE FAST Universal 2X qPCR Master Mix (2 x 5 mL = 10 mL) | For 1000 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). |
KK4705 | KAPA PROBE FAST ABI Prism® 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4706 | KAPA PROBE FAST ABI Prism® 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4707 | KAPA PROBE FAST ABI Prism® 2X qPCR Master Mix (2 x 5 mL = 10 mL) | For 1000 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4709 | KAPA PROBE FAST Bio-Rad iCycler 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. |
KK4710 | KAPA PROBE FAST Bio-Rad iCycler 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. |
KK4711 | KAPA PROBE FAST Bio-Rad iCycler 2X qPCR Master Mix (2 x 5 mL =10 mL) | For 1000 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. |
KK4716 | KAPA PROBE FAST ROX Low 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KK4717 | KAPA PROBE FAST ROX Low 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KK4718 | KAPA PROBE FAST ROX Low 2X qPCR Master Mix (2 x 10 mL) | For 1000 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KAPA PROBE FAST qPCR Kits are designed for high throughput, fast-cycling, real-time PCR using sequence-specific fluorogenic probes. These kits are compatible with all fluorogenic probe-based technologies, including hydrolysis probes (e.g. TaqMan®) and displacement probes (e.g. molecular beacons).
The KAPA PROBE FAST qPCR Master Mix (2X) is a ready-to-use cocktail containing all components except primers, probe(s) and template for fast cycling probe-based real-time PCR. The 2X Master Mix contains KAPA Taq HotStart DNA polymerase, KAPA PROBE FAST qPCR Buffer, dNTPs, MgCl2 and stabilizers.
KAPA Taq HotStart DNA Polymerase is an antibody-mediated hot start formulation of KAPA Taq DNA polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.
A total of 168 human genomic DNA samples were successfully genotyped along with 24 no-template controls using an ATP1B3 SNP genotyping assay on the ABI 7900HT real-time PCR system. Reaction volumes also included human genomic DNA (10 ng), 200 nM of each primer, and 200 nM of each hydrolysis probe (Allele X – FAM/ BHQ®-1, Allele Y – VIC/ BHQ-1) using the following standard cycling protocol: 95ºC for 10 minutes followed by 40 cycles of 95ºC, 15 seconds; 60ºC, 60 seconds.
Results for all 5 amplicons across a 5 point dilution series were obtained when assayed in penta-plex using a fast cycling protocol. Reaction volumes also included human genomic DNA (0.39 ng – 100 ng), 200 nM of each primer, and 200 nM of each hydrolysis probe (ACTB – FAM™/BHQ®-1, ERBB2 – CAL Fluor® Gold 540/ BHQ-1, ERBB3 – CAL Fluor Orange 560/ BHQ-2, EGFR – CAL Fluor Red 610/ BHQ-2, ACTG1 – Quasar® 705/ BHQ-2) using the following fast cycling protocol: 95ºC for 3 minutes followed by 40 cycles of 95ºC, 15 seconds; 60ºC, 15 seconds.
Results for serial dilutions of low copy number sample of similar abundance levels were obtained using a fast cycling protocol. Reaction volumes included human genomic DNA (1.5-fold dilutions over a 89 – 450 copies per reaction range), 200 nM of each primer, and 200 nM of hApoB100 (FAM/ BHQ®-1) hydrolysis probe using a fast cycling protocol: 95ºC for 3 minutes followed by 40 cycles of 95ºC, 3 seconds; 60ºC, 20 seconds.
Amplification plots were generated using 10-fold dilutions of a synthetic oligonucleotide target (60 – 6 x 106 copies per reaction) tested in triplicate. Reaction volumes also included 200 nM of each primer, and 200 nM of hApoB100 (FAM/ BHQ®-1) hydrolysis probe using the following fast cycling protocol: 95ºC for 3 minutes followed by 40 cycles of 95ºC, 3 seconds; 60ºC, 30 seconds.
Amplification plots were generated using either a standard cycling protocol (95ºC for 10 minutes followed by 40 cycles of 95ºC, 15 seconds; 60ºC, 60 seconds) or a fast cycling protocol: 95ºC for 3 minutes followed by 40 cycles of 95ºC, 3 seconds; 60ºC, 20 seconds. Reaction volumes also included human genomic DNA (10-fold dilutions over a 0.1 ng – 100 ng per reaction range), 200 nM of each primer, and 200 nM of hApoB100 (FAM/ BHQ®-1) hydrolysis probe.
Certain applications of this product are covered by patents issued to parties other than Kapa Biosystems and applicable in certain countries. Purchase of this product does not include a license to perform any such applications. Users of this product may therefore be required to obtain a patent license depending upon the particular application and country in which the product is used.
A license to perform the patented 5’ Nuclease Process for research is obtained by the purchase of (i) both Authorized 5′ Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5’ Nuclease Kit, or (iii) license rights from Applied Biosystems.
This product is an Authorized 5’ Nuclease Core Kit. Use of this product is covered by one or more of the following claims outside the US corresponding to US Patent No. 5,210,015, 5,487,972, and 5,928,907 (claim numbers 12-24, 27-28). The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. Separate purchase of a Licensed Probe would convey rights under US Patents and corresponding patent claims outside the US: 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and and claims outside the United States corresponding to US Patent No. 6,214,979. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Licensed under U.S. Patent nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.
iCycler®, Mx3000P®, Mx3005™, Mx4000®, Rotor-Gene™, DNA Engine Opticon™, Chromo 4™, LightCycler® , Smart Cycler®, ROX and TaqMan® are trademarks or registered trademarks of their respective companies.