KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.
KK4300 | KAPA Probe Force qPCR 100 x 20 µL reactions (Universal) | 1 ml |
KK4301 | KAPA Probe Force qPCR 500 x 20 µL reactions (Universal) | 5 ml |
KK4302 | KAPA Probe Force qPCR 1000 x 20 µL reactions (Universal) | 10 ml |
KK4303 | KAPA Probe Force qPCR 5000 x 20 µL reactions (Universal) | 50 ml |
Direct qPCR from crude extracts. Competing master mixes used in traditional blood, tissue, and plant qPCR workflows require robust upstream sample processing (e.g. column purification or nuclease digestion). KAPA PROBE FORCE enables the use of rapid crude DNA extraction methods, significantly reducing overall workflow time and cost.
High efficiency target amplification. Reaction efficiencies achieved for inhibitor spiked samples were examined and compared to that of purified DNA. Across various inhibitor types, efficiencies remained within 90-110%.
Broad range of high inhibitor resistance. KAPA PROBE FORCE exhibits consistent and robust amplification across all inhibitors tested, without observable Cq delays. Baseline performance of KAPA PROBE FORCE and competing master mixes was measured by creating standard curves with purified DNA according to each manufacturer’s recommended cycling conditions. Serial dilutions were run in the following ranges: Human: 100ng-10pg, Mouse: 100ng-10pg, Plant: 25ng-8pg, Bacteria: 2ng-2fg. Inhibitors were individually spiked into purified DNA samples at high concentrations to determine their effect on Cq values.
Crude sample duplex SNP detection. KAPA PROBE FORCE provides accurate genotyping and tight clustering in the presence of crude extracts (A) cotton seed, 0.5M NaOH extraction, (B) grapevine leaf, 75 mM Tris-HCL & 5 mM TCEP extraction for rapid SNP analysis.