KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. Kits contain a novel DNA polymerase engineered through a process of molecular evolution – the result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry.
The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity. The KAPA SYBR® DNA Polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC-rich and AT-rich templates.
↓ KAPA SYBR FAST Universal TDS
↓ KAPA SYBR FAST LightCycler 480 TDS
KK4600 | KAPA SYBR® FAST Universal 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). |
KK4601 | KAPA SYBR® FAST Universal 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). |
KK4602 | KAPA SYBR® FAST Universal 2X qPCR Master Mix (2 x 5 mL =10 mL) | For 1000 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each). |
KK4603 | KAPA SYBR® FAST ABI Prism® 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4604 | KAPA SYBR® FAST ABI Prism® 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4605 | KAPA SYBR® FAST ABI Prism® 2X qPCR Master Mix (2 x 5 mL =10 mL) | For 1000 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4606 | KAPA SYBR® FAST Bio-Rad iCycler 2X qPCR Master MIx (1 mL) | For 100 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. |
KK4607 | KAPA SYBR® FAST Bio-Rad iCycler 2X qPCR Master MIx (5 mL) | For 500 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. |
KK4608 | KAPA SYBR® FAST Bio-Rad iCycler 2X qPCR Master MIx (2 x 5 mL =10 mL) | For 1000 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers. |
KK4609 | KAPA SYBR® FAST Roche LightCycler® 480 2X qPCR Master Mix (1 mL) | For 100 x 20 µL reactions. Optimized for use with the Roche LightCycler® 480. |
KK4610 | KAPA SYBR® FAST Roche LightCycler® 480 2X qPCR Master Mix (5 mL) | For 500 x 20 µL reactions. Optimized for use with the Roche LightCycler® 480. |
KK4611 | KAPA SYBR® FAST Roche LightCycler® 480 2X qPCR Master Mix (2 x 5 mL =10 mL) | For 1000 x 20 µL reactions. Optimized for use with the Roche LightCycler® 480. |
KK4617 | KAPA SYBR® FAST ABI Prism® 2X qPCR Master Mix (1 x 50 mL) | For 5000 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™. |
KK4618 | KAPA SYBR® FAST Universal 2X qPCR Master Mix (1 x 50 mL) | For 5000 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (2 mL each). |
KK4619 | KAPA SYBR® FAST ROX Low 2X qPCR Master Mix (1 x 1 mL) | For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KK4620 | KAPA SYBR® FAST ROX Low 2X qPCR Master Mix (1 x 5 mL) | For 500 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KK4621 | KAPA SYBR® FAST ROX Low 2X qPCR Master Mix (2 x 5 mL) | For 1000 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KK4622 | KAPA SYBR® FAST ROX Low 2X qPCR Master Mix (1 x 50 mL) | For 5000 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™. |
KAPA SYBR® FAST qPCR Kits are supplied as a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR.
The KAPA SYBR® FAST qPCR Kit is supplied as a 2x Master Mix with integrated antibody-mediated hot start, SYBR® Green I fluorescent dye, MgCl2, dNTPs, and stabilizers. ROX reference dye is included in the ABI Prism® Master Mix (2X), and supplied in separate tubes in the Universal Master Mix (2X). Fluorescein reference dye is included in the Bio-Rad iCycler Master Mix (2x). The KAPA SYBR® FAST qPCR Master Mix (2X) optimized for the Roche LightCycler® 480 does not contain reference dye.
KAPA SYBR® FAST qPCR Kits are intended for use on all real-time instruments and protocols.
KAPA SYBR® FAST qPCR Kits are ideally suited for:
No bias in amplification efficiency across a wide range of GC contents (44.2 – 62.5%) was observed with KAPA SYBR FAST. Amplification efficiencies achieved for ten housekeeping genes with the KAPA SYBR FAST Universal qPCR Kit were plotted against GC content. The reaction efficiency achieved for each of the ten genes fell within the optimal range of 95% – 105%, independent of the nature of the amplicon.
Two target genes were amplified using a 10-fold dilution series of human genomic DNA (16 ng to 1.6 pg per 20 µL reaction) comparing four commercial kits. KAPA SYBR FAST consistently provides high performance for accurate expression quantitation across a range of difficult targets.
A 150 bp fragment of the human coagulation factor V gene was amplified from a set of five 10-fold dilutions of human genomic DNA (20 ng to 2 pg) with a standard-cycling protocol (green) and a fast-cycling protocol (blue).
Certain applications of this product are covered by patents issued to parties other than Kapa Biosystems and applicable in certain countries. Purchase of this product does not include a license to perform any such applications. Users of this product may therefore be required to obtain a patent license depending upon the particular application and country in which the product is used.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056, 6,171,785, and 5,928,907 (claim numbers 12-24, 27-28). The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
This product is provided under an agreement between Molecular Probes, Inc. and Kapa Biosystems Inc., and the manufacture, use, sale or import of this product is subject to one or more of U.S. Patent Nos. 5,436,134; 5,658,751 and corresponding international equivalents, owned by Molecular Probes, Inc. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Molecular Probes, Inc., Business Development, 29851.
Licensed under U.S. Patent nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.
The purchase of this product includes a limited, non-transferable license under specific claims of U.S. Patent Nos. 6,174,670, 6,569,627 and 5,871,908, owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology, Inc. and Roche Diagnostics GmbH, to use only the enclosed amount of product according to the specified protocols. No right is conveyed, expressly, by implication, or by estoppel, to use any instrument or system under any claim of U.S. Patent Nos. 6,174,670, 6,569,627 and 5,871,908, other than for the amount of product contained herein.
SYBR® is a registered trademark of Molecular Probes, Inc, Oregon. PRISM® and GeneAmp® are registered trademarks of Applera Corporation. iCycler®, Mx3000P®, Mx3005™, Mx4000®, Roto-Gene™, DNA Engine Opticon™, Chromo 4™, LightCycler® , and Smart Cycler® are trademarks or registered trademarks of their respective companies.