PCRBIO VeriFi & HS VeriFi Hifi Polymerases and Ready Mix Kits

PCRBIO VeriFi Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required. Enhanced processivity combined with advanced buffer chemistry give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

Amplification of a 13.5kb fragment of the human ß-globin gene at different annealing temperatures (A: 68.5°C, B: 66.0°C, C: 63.0°C, D: 60.5°C). The starting template amount is 30ng human genomic DNA. GC content is 37%. Reactions were set up using master mix formats (apart from Takara’s PrimeSTAR® GXL DNA Polymerase) and following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 95°C 15 sec, annealing 15 sec, 72°C 12 min.

AptaLock™ hot start technology
PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits both the 3’-5’ exonuclease activity and 5’- 3’ polymerase activity of the enzyme at ambient temperatures. This unique hot start molecule prevents primer dimer formation and nonspecific amplification to maximise the sensitivity and specificity of your PCR. This feature makes PCRBIO HS VeriFi™ Polymerase highly suitable for multiplexing and enables reactions to be set up at room temperature, with benchtop stability both before and after PCR for up to 24 hours.

Features 

• 100x higher fidelity than Taq DNA polymerase
• Increased PCR success rates with complex genomic templates (17.5kb and over)
• High yields under standard and fast PCR conditions (10-30s/kb)
• High temperature cycling for reduced GC bias
• Efficient and specific amplification from challenging templates including GC and AT rich sequences
• Advanced buffer chemistry including Mg and dNTPs
• Generates blunt-end PCR products
• Available as a convenient 2x ready mix with the option of a red dye for direct gel loading