PCRBIO VeriFi Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required. Enhanced processivity combined with advanced buffer chemistry give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
Amplification of a 13.5kb fragment of the human ß-globin gene at different annealing temperatures (A: 68.5°C, B: 66.0°C, C: 63.0°C, D: 60.5°C). The starting template amount is 30ng human genomic DNA. GC content is 37%. Reactions were set up using master mix formats (apart from Takara’s PrimeSTAR® GXL DNA Polymerase) and following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 95°C 15 sec, annealing 15 sec, 72°C 12 min.
AptaLock™ hot start technology
PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits both the 3’-5’ exonuclease activity and 5’- 3’ polymerase activity of the enzyme at ambient temperatures. This unique hot start molecule prevents primer dimer formation and nonspecific amplification to maximise the sensitivity and specificity of your PCR. This feature makes PCRBIO HS VeriFi™ Polymerase highly suitable for multiplexing and enables reactions to be set up at room temperature, with benchtop stability both before and after PCR for up to 24 hours.
|PB10.42-01||PCRBIO VeriFi Polymerase||100 units|
|PB10.42-05||PCRBIO VeriFi Polymerase||500 units|
|PB10.43-01||PCRBIO VeriFi Mix||100 reactions|
|PB10.43-05||PCRBIO VeriFi Mix||500 reactions|
|PB10.44-01||PCRBIO VeriFi Mix Red||100 reactions|
|PB10.44-05||PCRBIO VeriFi Mix Red||500 reactions|
|PB10.45-01||PCRBIO HS VeriFi Polymerase||100 units|
|PB10.45-05||PCRBIO HS VeriFi Polymerase||500 units|
|PB10.46-01||PCRBIO HS VeriFi Mix||100 reactions|
|PB10.46-05||PCRBIO HS VeriFi Mix||500 reactions|
|PB10.47-01||PCRBIO HS VeriFi Mix Red||100 reactions|
|PB10.47-05||PCRBIO HS VeriFi Mix Red||500 reactions|