Gel Cassettes are pre-cast with agarose, and use ethidium bromide to visualize DNA and provide more uniform migration of fragments. Cassettes have a capacity to run 4 samples and are shipped in boxes of 10 each. Loading dye and DNA marker (for calibration) sufficient for all 40 runs are provided with each shipment.
Gel cassettes are available for collections of DNA within the following target ranges:
3% Agarose → for targets between 50 bp – 200 bp
2% Agarose → for targets between 100 bp – 600 bp
1.5% Agarose → for targets between 300 bp – 1.5 kb
0.75% Agarose → for targets between 2 kb – 8 kb (Pippin Prep) and 1 kb – 50 kb (BluePippin)
PippinPrep | ||
CDF1510 | 1.5% Agarose, dye-free, w/ internal standards, Pippin Prep, 250 bp -1.5kb | 10/Pk |
CDF2010 | 2% Agarose, dye-free, w/ internal standards, Pippin Prep, 100 – 600 bp | 10/Pk |
CDF3010 | 3% Agarose, dye-free, w/ internal standards, Pippin Prep, 90-250 bp | 10/Pk |
CEF2010 | 2% agarose, ethidium-free. Extracts targets between 100 – 600 bp | 10/Pk |
CSD1510 | 1.5% agarose with ethidium bromide. Extracts targets between 0.3 – 1.5 kb | 10/Pk |
CSD2010 | 2% agarose with ethidium bromide. Extracts targets between 100 – 600 bp | 10/Pk |
CSD3010 | 3% agarose, with ethidium bromide. Extracts targets between 90 – 250 bp | 10/Pk |
CSD7510 | 0.75% agarose with ethidium bromide. Extracts targets between 2 – 8 kb | 10/Pk |
SSQ2010 | 2% agarose with ethidium bromide. For use with ScriptSeq Complete™ kits from Epicentre | 10/Pk |
SDF2010 | 2% Agarose, dye-free, w/ internal standards, SEM – Small Elution module, 100 – 600 bp | 10/Pk |
BluePippin | ||
BLG7510 | 0.75% Agarose, band capture w/ Midori Green, BluePippin, 2-10 kb | 10/Pk |
BSG1510 | 1.5% Agarose, band capture with Midori Green, BluePippin, 250 bp – 1.5 kb | 10/Pk |
BLF7510 | 0.75% Agarose, dye-free w/ external markers, BluePippin, low range, 1-10 kb | 10/Pk |
BUF7510 | 0.75% Agarose, dye-free w/ external markers, BluePippin, mid range, 10-18 kb | 10/Pk |
PAC20KB | 0.75% Agarose, Dye Free, PacBio ~20kb templates, S1 | 10/Pk |
BMF7510 | 0.75% Agarose, dye-free w/ external markers, BluePippin, high range, 18-27 kb | 10/Pk |
BDF3010 | 3% Agarose, dye-free, w/ internal standards, BluePippin, 90-200 bp | 10/Pk |
BDF1510 | 1.5% Agarose, dye-free, w/ internal standards, BluePippin, 250 bp -1.5 kb | 10/Pk |
BDF2010 | 2% Agarose, dye-free, w/ internal standards, BluePippin, 100 – 600 bp | 10/Pk |
BEF2010 | 2% Agarose, dye-free, w/external standards, BluePippin, 100 – 600 bp | 10/Pk |
BHF7510 | 0.75% Agarose, dye-free w/external standards, BluePippin, 40, or 50 kb | 10/Pk |
BSQ2010 | 2% Agarose, dye-free for use with Epicentre ScriptSeq Complete™ kits | 10/Pk |
BSF2010 | 2% Agarose, dye-free, w/ internal standards, low elution-25ul, BluePippin, 100 – 600 bp | 10/Pk |
Running a gel cassette is quite simple, and requires just a couple minutes per sample. A brief overview of the workflow:
1. Bring DNA up to 30ul in TE
2. Add 10ul of loading solution
3. Remove a cassette from its foil bag
4. Place cassette on instrument nest and remove two strips of adhesive tape
5. Remove 40ul of buffer from a sample well, and add 40ul of sample, repeat
6. Remove 40ul of buffer from the remaining well, and load 40ul of DNA marker
7. Close lid
8. Enter DNA collection range for each sample in software
9. Press run
10.When complete, remove fractions from collection wells with a pippette