The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR.
KAPA Long Range DNA Polymerase possesses 3x – 4x higher fidelity than Taq polymerase. The majority of PCR products from a KAPA Long Range HotStart DNA Polymerase reaction have a single deoxyadenosine added to the 3’ ends of the amplicon and can be used for ligation into single 3’-T -overhang cloning sites.
The KAPA Long Range PCR system is ideally suited for:
↓ KAPA Long Range HotStart Brochure
KK3005 | KAPA Long Range DNA Polymerase with dNTPs (250 U) | 250 units, 5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK3006 | KAPA Long Range DNA Polymerase with dNTPs (100 U) | 100 units, 5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK3501 | KAPA Long Range HotStart DNA Polymerase with dNTPs (100 U) | 100 units, 2.5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK3502 | KAPA Long Range HotStart DNA Polymerase with dNTPs (250 U) | 250 units, 2.5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK3503 | KAPA Long Range HotStart DNA Polymerase with dNTPs (500 U) | 500 units, 2.5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK3601 | KAPA Long Range HotStart ReadyMix with dye (100 x 25 µL reactions) | Kit includes 100 x 25 µl reactions of KAPA Long Range HotStart ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA Long Range HotStart DNA Polymerase, reaction buffer, Mg2+, and loading dye. |
KK3602 | KAPA Long Range HotStart ReadyMix with dye (500 x 25 µL reactions) | Kit includes 500 x 25 µl reactions of KAPA Long Range HotStart ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA Long Range HotStart DNA Polymerase, reaction buffer, Mg2+, and loading dye. |
The KAPA Long Range system polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleotide triphosphates. Both enzymes possess 5’→3’ polymerase activity, but only Taq possesses double strand dependent 5’→3’ exonuclease activity and only the Type B polymerase possesses 3’→5’ exonuclease (proofreading) activity. KAPA Long Range possesses 3x – 4x higher fidelity than Taq polymerase. KAPA HiFi DNA Polymerase is recommended for short and mid-range targets where higher fidelity is required.
All KAPA Long Range kits are supplied with 5x KAPA Long Range Buffer and 250 µl KAPA dNTPs (10 mM each). This buffer has been optimized for demanding applications that require long range and high sensitivity. Magnesium chloride solution is supplied separately to accommodate PCR optimization.
The KAPA Long Range PCR system is suited for:
Amplification of template dilution of human genomic DNA starting with 50 ng, 10 ng, 2 ng, 400 pg per 25 µL. Amplicons were 737 bp, 1.3 kb, 3.3 kb, and 4.5 kb in lengths. 35 cycles, 72˚C extension temperature.
Amplification of template dilution of human genomic DNA starting with 50 ng, 10 ng, 2 ng, 400 pg per 25 µL. Amplicons were 7.5 kb, 10.5 kb, 13 kb, and 15 kb in lengths. 35 cycles, 68˚C extension temperature.
Amplification of 4.5 kb fragment from 50 ng, 10 ng, 2 ng, and 400 pg of human genomic DNA per 25 µL reaction. Lanes: (M) Marker, (1-4) KAPA Long Range HotStart, (5-8) Competitor T, (13-16) Competitor R.