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VeriFi™ Library Amplification Mix

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VeriFi™ Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.

A superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias1. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads2. The mix can be combined with our library quantification kit during NGS library preparation.

Features

  • Low GC bias, ideal for high GC/AT targets
  • More unique reads per NGS dataset for superior data quality
  • AptaLock™ hot start technology for maximum sensitivity and specificity
  • 100x higher fidelity than Taq DNA polymerase
  • Room temperature setup
  • 2x ready mix for minimal pipetting

 

  • VeriFi™ Library Amplification Mix was created as a tool for researchers conducting next generation sequencing (NGS) studies. A key part of PCR-based NGS workflows is the library amplification step during which a DNA or cDNA (in the case of RNA-Seq studies) library has been modified to contain appropriate adaptors for the intended sequencing platform and needs to be amplified before proceeding with the sequencing step.

    To ensure accuracy of the final sequencing data, proofreading polymerases used in the library amplification step of an NGS pipeline must have certain key characteristics, which are:

    • High Fidelity, to minimise PCR errors in nucleotide incorporation.
    • Strong multiplexing capability, to amplify varying fragment lengths in a library.
    • Low sequence-dependent bias, particularly when dealing with complex (GC/AT-rich, or repetitive) sequences.

     

    Additional features can also help improve library amplification mix performance, depending on the type of NGS study being undertaken. VeriFi™ Library Amplification Mix is engineered with these features in mind. It is powered by our market leading VeriFi™ Polymerase, with AptaLock™ hot start technology, in a novel 2x mix format. The mix contains reaction buffer, dNTPs, Mg2+, and enhancers that minimise GC-bias and enable reliable library amplification for Illumina® sequencing platforms. Amplified libraries can easily be quantified using NGSBIO Library Quant Kit Blue for Illumina® prior to sequencing.

    Better quality NGS data

    VeriFi™ Library Amplification Mix was externally validated using Illumina®-based sequencing in a double-blind comparison with three market-leading competitors. VeriFi™ Library Amplification Mix consistently gave 2% more uniquely mapped reads than competitors for three different microbial genomes. Depending on sequencing platform being used, this small percentage can translate to 80,000 to 100,000,000 more unique reads. This leads to greater target sequence coverage and more accurate quantitative information, making sequencing experiments more cost-effective and resulting data more informative.

    Reduced GC bias during NGS library amplification

    Novel buffer composition makes VeriFi™ Library Amplification Mix ideal for complex templates. Our research team has challenged this enzyme in PCRs on numerous difficult templates, demonstrating minimal bias during amplification at extremes of GC content ranging from 30% – 70%. With consistently better performance than all major competitors, these results ensure cutting edge performance of the mix regardless of the target sequence, to give you confidence when undertaking costly and laborious NGS experiments.

    AptaLock™ hot start technology

    VeriFi™ Library Amplification Mix is enhanced by PCRBIO’s innovative AptaLock™ hot start technology, a proprietary aptamer-like molecule that reversibly inhibits both the 3’-5’ exonuclease activity and 5’-3’ polymerase activity of the enzyme at ambient temperatures. The unique hot start molecule prevents primer dimer formation and non-specific amplification to maximise PCR sensitivity and specificity. This ensures VeriFi™ Library Amplification Mix reactions can be set up at room temperature.

  • ↓ VeriFi™ Library Amplification Mix – PCR Biosystems

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OUR MOTIVATION

Many established technology providers learned the hard way that having a worldwide direct sales force, is often not the best solution. Now that the focus might have shifted to other continents, the cost of local affiliates weigh heavily on their organizations. For new and upcoming players, the investments are too high and the local barriers too complex. For innovative suppliers in general, the typical resellers and large catalog companies will lack the scientific knowhow and focus in order to be successful. New innovative suppliers need to have a fast and efficient access to the world. As the Life Science part of the Aptco Invest group of companies, the Sopachem team tries hard to redefine the meaning of the word 'distributor'.

OUR VISION

A local partner willing to go the extra mile, can offer the best of both worlds. We envisioned that by delivering more than what is expected of a distributor, it is possible to become a true local sales and marketing partner for our suppliers. With sales and service engineers trained to the same level of a direct sales force, both the customers and suppliers win. For our instrumentation suppliers, a strong service team is an essential driver for success. For reagents and consumables, we can ensure next-day deliveries to our endusers thanks to our centralized operations with local stock.

OUR MISSION

Serving laboratories in the Benelux, Sopachem thrive to be such kind of distributor. To maintain our focus, we created several divisions, each serving their specific markets, each with team of high level product and application specialist behind them. For our suppliers, our mission is to be the best sales and marketing partner available in our region, bringing the word distributor to another level. For our customers, it is our mission to serve them with highest standards of product knowhow, understanding their needs and applications. Be it by offering the best service or by introducing innovative technologies that will in the end help them advance their scientific work.
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