SYGNIS TruePrime Single Cell WGA Kits
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The Sygnis TruePrime™ Single Cell WGA kit uses a revolutionary novel multiple displacement amplification (MDA) method based on the combination of the recently discovered TthPrimPol and Phi29 DNA polymerase for the amplification of genomic DNA from single cells.
- Primer-free method: TthPrimPol synthesizes the primers for Phi29 DNA pol.
- No primer artefacts: The absence of random synthetic primers prevents any amplification artefacts generated by random extension of primer dimers, etc.
- Insensitive to external DNA contaminations.
- Easy and reliable.
- Exquisite reproducibility when amplifying from single mammalian cells.
- Reduced amplification bias in genome coverage compared to methods based on random synthetic primers.
- Ideally suited for next generation sequencing, tested for Illumina and Ion Torrent workflows.