KAPA2G Fast DNA Polymerase is a second-generation enzyme derived through a process of molecular evolution. The KAPA2G Fast DNA Polymerase was engineered for higher processivity and speed, offering significantly faster extension rates and improved PCR success rates than wild-type Taq polymerase. KAPA2G Fast is well suited for both routine and high-throughput PCR, achieving higher yields and improved sensitivity than competitor enzymes across a wide range of amplicon types.
KAPA2G Fast DNA Polymerase and optimized buffer system offers:
KK5008 | KAPA2G Fast DNA Polymerase with dNTPs (100 U) | 100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM). |
KK5009 | KAPA2G Fast DNA Polymerase with dNTPs (250 U) | 250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM). |
KK5020 | KAPA2G Fast DNA Polymerase (100 U) | 100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). |
KK5021 | KAPA2G Fast DNA Polymerase (250 U) | 250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). |
KK5101 | KAPA2G Fast ReadyMix with dye (100 x 25 µL reactions) | Kit includes 100 x 25 µl reactions of KAPA2G Fast ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA2G Fast DNA Polymerase, reaction buffer, Mg2+, and loading dye. |
KK5102 | KAPA2G Fast ReadyMix with dye (500 x 25 µL reactions) | Kit includes 500 x 25 µl reactions of KAPA2G Fast ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA2G Fast DNA Polymerase, reaction buffer, Mg2+, and loading dye. |
KK5500 | KAPA2G Fast HotStart DNA Polymerase with dNTPs (500 U) | 500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM). |
KK5501 | KAPA2G Fast HotStart DNA Polymerase (500 U) | 500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). |
KK5502 | KAPA2G Fast HotStart DNA Polymerase with dNTPs (250 U) | 250 U, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM). |
KK5503 | KAPA2G Fast HotStart DNA Polymerase (250 U) | 250 units, 5 U/µL. Supplied with proprietary 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). |
KK5510 | KAPA2G Fast HotStart DNA Polymerase with dNTPs – Mg2+ free buffer (500 U) | 500 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M, dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK5511 | KAPA2G Fast HotStart DNA Polymerase – Mg2+ free buffer (500 U) | 500 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M and MgCl2 (25 mM). |
KK5512 | KAPA2G Fast HotStart DNA Polymerase with dNTPs – Mg2+ free buffer (250 U) | 250 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M, dNTP Mix (10 mM) and MgCl2 (25 mM). |
KK5513 | KAPA2G Fast HotStart DNA Polymerase – Mg2+ free buffer (250 U) | KAPA2G Fast HotStart DNA Polymerase.^(250 U, 5 U/µL). With Mg-free proprietary 5X KAPA2G Buffer M and MgCl2 (25 mM). |
KK5519 | KAPA2G Fast HotStart DNA Polymerase (2,500 U) | 2500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). |
KK5523 | KAPA2G Fast HotStart DNA Polymerase (100 U) | 100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM). |
KK5530 | KAPA2G Fast HotStart DNA Polymerase with dNTPs (100 U) | 100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM). |
KK5601 | KAPA2G Fast HotStart ReadyMix (500 x 25 µL rxns) | KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM. |
KK5603 | KAPA2G Fast HotStart ReadyMix (100 x 25 µL rxns) | KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM. |
KK5608 | KAPA2G Fast HotStart ReadyMix with dye (100 x 25 µL reactions) | KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format with loading dye. Contains Mg2+ at a 1X conc. of 1.5 mM. |
KK5609 | KAPA2G Fast HotStart ReadyMix with dye (500 x 25 µL reactions) | KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format with loading dye. Contains Mg2+ at a 1X conc. of 1.5 mM. |
KAPA2G Fast DNA Polymerase, a second-generation enzyme derived through a process of molecular evolution. KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.
KAPA2G Fast HotStart is particularly well suited for high throughput Fast PCR, offering total reaction times 20 – 70% shorter than conventional PCR assays performed with wild-type Taq polymerase or hot start formulations thereof. In addition, KAPA2G Fast HotStart has been shown to achieve higher yields and sensitivity than competitor enzymes across a wide range of amplicon types. No specialized PCR consumables or thermocyclers are required.
KAPA2G Fast DNA Polymerase and optimized buffer system offers:
Any standard, end-point PCR assay performed efficiently with wild-type Taq polymerase (or a hot start formulation thereof) may be converted to a Fast PCR assay with KAPA2G Fast HotStart. Amplicons generated with KAPA2G Fast HotStart are suitable for routine downstream applications, including restriction enzyme digestion, cloning and sequencing.
Total PCR times for the generation of different sized amplicons for human genomic DNA (≤3.5 kb) and lambda (≤5 kb) using wild-type Taq DNA polymerase and KAPA2G Fast DNA Polymerase. The amount of time that can be saved using protocols based on wild-type Taq is limited by the extension rate of the enzyme. KAPA2G Fast DNA Polymerase is based on a second-generation polymerase with an ability to synthesize DNA faster than wild-type Taq or other DNA polymerases. Reaction times are based on a 35-cycle program using the cycling profile recommended by each kit manufacturer.
High speed and performance on KAPA2G FAST